DNA-Prep Reagents Kit, RUO

Cell cycle assay human - HeLa

Experiment
Cell cycle assay human - HeLa
Product
DNA-Prep Reagents Kit, RUO from Beckman Coulter
Manufacturer
Beckman Coulter

Protocol tips

Upstream tips
- cells were seeded at 5×104 cells/ml and treated with IC50 values of AFha, AFe and Cha and incubated for 16 and 32 h
Protocol tips
- 100 µl lysis reagent were added to each tube and incubated for 5 min at room temperature.

Publication protocol

HeLa cells were seeded at 5×104 cells/ml in 10 ml aliquots in 10 cm culture dishes and treated with IC50 values of AFha, AFe and Cha. The cells were incubated for 16 and 32 h. After the appropriate incubation period, the HeLa cells were trypsinized for 10 min, re-suspended in phosphate-buffered saline (PBS) and transferred to polypropylene tubes. The Coulter DNA Prep reagents kit was used for DNA cell cycle analysis, as per the manufacturer's instructions. Briefly, 100 µl lysis reagent were added to each tube and incubated for 5 min at room temperature. Thereafter, 500 µl PI (50 µg/ml) were added and the tubes were incubated for 15 min at 37°C. Flow cytometric analysis was performed directly following incubation. A Beckman Coulter Cytomics FC500 was used for all flow cytometricanalysis. FlowJo_V10 was used for analysis.

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Discussion

Discussion

3 years ago

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Passage number

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?

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Papers

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Paper title
Anticancer potential of selected Adans species
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Manufacturer protocol

Download the product protocol from Beckman Coulter for DNA-Prep Reagents Kit, RUO below.

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