β-Gal Reporter Gene Assay, chemiluminescent

Protocol tips

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	Fix cells for 15 minutes in a fixative solution Add 1 ml of the Staining Mixture per well and incubate overnight at 37 °C without CO2 until the cells are stained blue

Protocol tips
Fix cells for 15 minutes in a fixative solution Add 1 ml of the Staining Mixture per well and incubate overnight at 37 °C without CO2 until the cells are stained blue

Publication protocol

Senescence-associated β-galactosidase activity was measured using a senescent staining kit (Sigma–Aldrich), and the treatment regimen for each group was the same as described above. Diabetic-mouse-derived MSCs were washed twice with phosphate-buffered saline and fixed for 15 minutes in a fixative solution. After washing with phosphate-buffered saline, fresh β-galactosidase staining solution was added and cells were incubated overnight at 37°C without CO2. Senescent cells displayed a β-galactosidase positive blue color in the cytoplasm.

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