EZ DNA Methylation-Gold Kit

DNA methylation profiling Gene specific profiling - SiHa TIMP2

Experiment
DNA methylation profiling Gene specific profiling - SiHa TIMP2
Product
EZ DNA Methylation-Gold Kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Protocol tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Methylation specific PCR (MSP)
DNA was isolated using standard phenol:chloroform extraction and quantified using an ND1000 spectrophotometer (Thermo Scientific). DNA (1 μg) was subjected to bisulfite modification using EZ gold methylation kit (Zymo Research). Bisulfite-modified DNA was used for MSP of the TIMP2 gene with a set of primers (9) spanning regions 1919–1987 (−325 to −257), relative to the transcription start site. MSP was performed as detailed by Ivanova et al (9). The PCR products were analysed on 3% agarose gel. MSP was carried out in duplicate.

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Discussion

Discussion

1 year ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

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Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation-Gold Kit below.

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Videos

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