QuantiFluor® dsDNA System

DNA quantification Human - HEK 293

Experiment
DNA quantification Human - HEK 293
Product
QuantiFluor® dsDNA System from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Exposure to light will reduce the sensitivity of the assay. Store QuantiFluor® dsDNA Dye and working solution protected from light

Publication protocol

Quantitative measurement of isolated exosomal DNA
To reduce external DNA contamination, prior to DNA extraction, exosomes were treated with DNase I (Roche Inc.) and Exonuclease III (Takara Inc.), according to the manufacturers' instructions43. After heat inactivation, the exosomal DNA was purified by Proteinase K (Wako) treatment. The amount of dsDNA was determined using an Agilent High Sensitivity DNA kit (Agilent Technologies) or a QuantiFluor dsDNA System (Promega).

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Reviews

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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - HEK 293 using QuantiFluor® dsDNA System from Promega.

Paper title
Exosomes maintain cellular homeostasis by excreting harmful DNA from cells
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Manufacturer protocol

Download the product protocol from Promega for QuantiFluor® dsDNA System below.

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Videos

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