ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- cells were pre-treated with or without NAC (30 mM) for 1 h, -cells were further incubated in the absence or the presence of gemcitabine (10 mM) for 24 h	- with ROS/superoxide detection solution for 10 min at 37 C

Upstream tips
- cells were pre-treated with or without NAC (30 mM) for 1 h, -cells were further incubated in the absence or the presence of gemcitabine (10 mM) for 24 h
Protocol tips
- with ROS/superoxide detection solution for 10 min at 37 C

Publication protocol

Intracellular ROS concentration was determined by using total ROS/superoxide detection kit (Enzo Life Science, San Diego, CA) according to the manufacturer's instructions. In brief, after Miapaca-2 cells were pre-treated with or without NAC (30 mM) for 1 h, the cells were further incubated in the absence or the presence of gemcitabine (10 mM) for 24 h. Then, the cells were collected and incubated with ROS/superoxide detection solution for 10 min at 37 C. At least 50,000 stained cells were analyzed on a FACSCanto II system (Becton Dickinson, Bedford, MA) by using 490 nm and 525 nm wave lengths as excitation and emission wave lengths, respectively, for each determination.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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