CelLytic™ B Cell Lysis Reagent

Protein isolation Bacteria - Salmonella enterica

Experiment
Protein isolation Bacteria - Salmonella enterica
Product
CelLytic™ B Cell Lysis Reagent from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
- Bacteria are centrifuged and pellets are suspended in 1 ml of 1X CelLytic™ B cell lysis reagent (Sigma), containing 0.2 mg/ml lysozyme (RPI Corp.), 50 U/ml Benzonase® Nuclease (Sigma), and 25 μl of Protease Inhibitor Cocktail (Sigma).

Publication protocol

Wild type and ST-2W bacteria were grown overnight in LB Broth. Bacteria were centrifuged and pellets were suspended in 1 ml of 1X CelLytic™ B cell lysis reagent (Sigma), containing 0.2 mg/ml lysozyme (RPI Corp.), 50 U/ml Benzonase® Nuclease (Sigma), and 25 μl of Protease Inhibitor Cocktail (Sigma). Cell extracts were centrifuged and supernatants containing soluble proteins were removed for SDS-PAGE analysis.

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Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Salmonella enterica using CelLytic™ B Cell Lysis Reagent from Sigma-Aldrich.

Paper title
CD4 T cell persistence and function after infection is maintained by low-level peptide:MHCII presentation
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ B Cell Lysis Reagent below.

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Videos

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