Qproteome Bacterial Protein Prep Kit

Protein isolation Bacteria - Salmonella enterica

Experiment
Protein isolation Bacteria - Salmonella enterica
Product
Qproteome Bacterial Protein Prep Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- For soluble protein preparations from up to 4.5 liters of bacterial cell culture.

- Before use, Native Lysis Buffer must be supplemented with lysozyme and Benzonase® Nuclease.
Downstream tips
- Downstream applications are SDS-PAGE and mass spectrometry.

- Solubilization of inclusion bodies requires high concentrations of denaturants (e.g., 6M Guanidine HCl).

Publication protocol

The cultures were grown until mid-exponential phase (OD of 2.7), and the 50 ml volume of cultures were used for a total protein extraction by using Qproteome Bacterial Protein Prep kit (Qiagen). Disulfide bonds within proteins were reduced with 2-Mercaptoethanol and separated by SDS-PAGE gel electrophoresis.

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Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Bacteria - Salmonella enterica using Qproteome Bacterial Protein Prep Kit from Qiagen.

Paper title
A Comprehensive Assessment of the Genetic Determinants in Typhimurium for Resistance to Hydrogen Peroxide Using Proteogenomics
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Manufacturer protocol

Download the product protocol from Qiagen for Qproteome Bacterial Protein Prep Kit below.

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Videos

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