Magnetic mRNA Isolation Kit

RNA isolation / purification Tissue - Mouse Hippocampus

Experiment

RNA isolation / purification Tissue - Mouse Hippocampus

Product

Magnetic mRNA Isolation Kit from New England BioLabs

Manufacturer

New England BioLabs

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. - If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation.

Protocol tips
- If the lysate viscous than normal. Pass these cell or tissue lysates several times through a 21-gauge needle. - If the yield of mRNA is low Increase the ratio of beads to Lysis/Binding Buffer or add RNase-free Proteinase K to the sample lysate directly before the 10 minute binding incubation.

Publication protocol

RNA was barcoded, pooled and sequenced on a lane of a Hiseq2000 using 51bp paired end reads. mRNA-seq library construction was as follows: Total RNA quantity and integrity were assessed, using Quant-IT RiboGreen RNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and Agilent Tapestation 2200 R6K. mRNA enrichment was achieved by processing 5μg of total RNA using the Magnetic mRNA Isolation Kit from NEB (S1550S) with minor modifications. Generation of double stranded cDNA and library construction were performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina® (E7420L) according to manufacturer specifications.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Hippocampus using Magnetic mRNA Isolation Kit from New England BioLabs.

Paper title

The Architecture of Parent-of-Origin Effects in Mice

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Manufacturer protocol

Download the product protocol from New England BioLabs for Magnetic mRNA Isolation Kit below.

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