LC3B antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute primary antibody in 1% normal serum or BSA (in 1X PBS). Incubate coverslips with ~5 µ g/mL rabbit anti-LC3 primary antibody for 1 hour at room temperature (37°C is optional), or for 16 hours at 4°C.

Protocol tips
Dilute primary antibody in 1% normal serum or BSA (in 1X PBS). Incubate coverslips with ~5 µ g/mL rabbit anti-LC3 primary antibody for 1 hour at room temperature (37°C is optional), or for 16 hours at 4°C.

Publication protocol

The STAT3 pathway was detected as previously described (26). Total harvested cells were lysed to perform immunoblots as previously reported (27). Immunoblotting was subjected to semi-quantitative analysis using ImageJ software.

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Manufacturer protocol

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