The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For optimal results you may alter the PPC, cell density, temperature, and incubation time.	Infect the cells with 60ppc (particle per cell) for 24hour.

Upstream tips
For optimal results you may alter the PPC, cell density, temperature, and incubation time.
Protocol tips
Infect the cells with 60ppc (particle per cell) for 24hour.

Publication protocol

Cells were cultured on Lab-Tek II Chamber Slides (Nalgene Nunc International, Rochester, 154534). The PremoTM Autophagy Tandem Sensor RFP-GFP-LC3B Kit (Life Technologies, P36239) was used to monitor autophagy flux. The cells were treated, followed by fixation in a 4% formaldehyde solution. Fluorescent images were obtained using the Laser Scanning Microscope 700 (Carl Zeiss).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit below.

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