CYTO-ID® Autophagy detection kit

Autophagy assay cell type - Gaucher macrophages

Experiment
Autophagy assay cell type - Gaucher macrophages
Product
CYTO-ID® Autophagy detection kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Upstream tips
This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry
Protocol tips
- 2 µL of CYTO-ID® Green Detection Reagent and 1 µL of Hoechst 33342 Nuclear Stain for every 1 mL of 1X Assay Buffer or complete cell growth medium
Downstream tips
Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.

Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.

Publication protocol

Both control and GMs macrophages were cultured in chamber slides. After the different treatments, cells were washed twice with 1X assay buffer and stained using a Cyto‐ID Autophagy Detection Kit (Enzo life science, Farmingdale, NY, USA), according to the manufacturer's instructions. Cells were imaged by confocal microscopy using a 40X oil DIC objective.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Gaucher macrophages using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.

Paper title
Lysosomal storage and impaired autophagy lead to inflammasome activation in aucher macrophages
Full paper
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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

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