Anti-LC3 (Rat) pAb

Autophagy assay cell type - MLO-Y4

Experiment
Autophagy assay cell type - MLO-Y4
Product
Anti-LC3 (Rat) pAb from MBL international corporation
Manufacturer
MBL international corporation

Protocol tips

Protocol tips
Primary antibody dilution 1:1000

Publication protocol

Total and cytosolic extracts were obtained using a commercial kit (Active Motif, Carlsbad, CA, USA). The following antibodies were used for immunoblotting: rabbit polyclonal anti-LC3 antibody (1:1000) (Code n.PD014, MBL International Corporation, Woburn, MA, USA), mouse anti-p62/SQSTM1 antibody (1:250) (Code n.MAB8028, R&D Systems, Minneapolis, MN, USA),
and mouse anti-b-actin monoclonal antibody (1:5000) (Code n.A5441, Sigma-Aldrich) as an internal control. Anti-rabbit (1:2000) (Code n.7074, Chemicon, Temecula, CA, USA) and anti-mouse (1:10,000) (Code n.103001, BD Pharmingen, Franklin Lakes, NJ, USA) antibodies were used as peroxidase-conjugated secondary antibodies. Chemiluminescence was detected using luminol solution (ECL Plus, GE Healthcare Amersham, Milan, Italy). Immunoreactive bands were visualized with an exposure time of
15 minutes (Kodak XOMAT). LC3 conversion from LC3 formI (LC3-I, 18 kDa) to LC3 form II (LC3-II, 16 kDa), normalized by b-actin level, was our reported autophagy index.

Full paper   Login or join for free to view the full paper.

Reviews

Anti-LC3 (Rat) pAb from MBL international corporation has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - MLO-Y4 using Anti-LC3 (Rat) pAb from MBL international corporation.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from MBL international corporation for Anti-LC3 (Rat) pAb below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - MLO-Y4 using Anti-LC3 (Rat) pAb from MBL international corporation. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms