HyClone RPMI 1640 media: Liquid

Mammalian cell culture media THP-1

Experiment
Mammalian cell culture media THP-1
Product
HyClone RPMI 1640 media: Liquid from Cytiva
Manufacturer
Cytiva

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (FBS; HyClone) and 100 units penicillin, 0.1 mg streptomycin and 0.25 μg amphotericin B per ml

Publication protocol

HEK-293T cells, CHME3 cells and Murine Embryonic Fibroblasts (MEFs) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Life Technologies) while U1 (monocytic cell line latently infected with HIV-1), THP-1, and U937 cells were grown in RPMI (Roswell Park Memorial Institute) 1640 supplemented with 10% fetal bovine serum (FBS; HyClone) and 100 units penicillin, 0.1 mg streptomycin and 0.25 μg amphotericin B per ml at 37°C in the presence of 5% CO2 in a humified incubator.

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Papers

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Paper title
Suppressor of Cytokine Signaling 3 (SOCS3) Degrades p65 and Regulate HIV-1 Replication
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Manufacturer protocol

Download the product protocol from Cytiva for HyClone RPMI 1640 media: Liquid below.

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