DMEM/F-12

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	MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

Protocol tips

MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

Publication protocol

MCF-10A cells were cultured as described []. VSV-G pseudotyped retroviruses were generated, and MCF-10A cells were infected and selected as described [] using a VSV-GPG producer line []. VSV-G pseudotyped lentiviruses were produced by co-transfection of pLKO hairpin vectors with accessory plasmids in 293T cells. Hairpin-encoding lentiviruses were used to infect MCF-10A cells or superinfect CRB3-expressing cells, and stable cell lines were obtained by selection with puromycin (2 μg/ml) or dual selection with puromycin (2 μg/ml) and G418 (300 μg/ml), respectively.

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