Gibco™ DMEM, high glucose

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	Bovine Coronary Artery Endothelial Cells (BCAECs) and mouse skeletal muscle cell line (C2C12 myoblasts) were maintained at 37°C in an incubator with humidified atmosphere of 5 % CO2 and cultured in DMEM supplemented with 10 % FBS and 1 % antibiotic and antimitotic solution.

Protocol tips
Bovine Coronary Artery Endothelial Cells (BCAECs) and mouse skeletal muscle cell line (C2C12 myoblasts) were maintained at 37°C in an incubator with humidified atmosphere of 5 % CO2 and cultured in DMEM supplemented with 10 % FBS and 1 % antibiotic and antimitotic solution.

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Bovine Coronary Artery Endothelial Cells (BCAECs) were purchased from Cell Applications, Inc. (San Diego, CA, USA). BCAECs obtained from healthy bovine coronary arteries from 4 hearts were used in this study. A mouse skeletal muscle cell line (C2C12 myoblasts) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Both cell types were maintained at 37°C in an incubator with humidified atmosphere of 5 % CO2 and cultured in DMEM supplemented with 10 % FBS and 1 % antibiotic and antimitotic solution.



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