FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit

Protocol tips

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	cultured in FGM-3 medium, in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated

Protocol tips
cultured in FGM-3 medium, in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated

Publication protocol

Normal human ventricular cardiac fibroblasts from one donor were purchased from Lonza (Lonza Bioscience, Singapore). Cells were cultured in FGM-3 medium (Lonza Bioscience, Singapore) and in a humidified incubator at 90% N2, 5% O2, 5% CO2 at all times unless otherwise indicated. Microdevices were autoclave-sterilized, plasma-treated and coated with human fibronectin (Sigma-Aldrich, Singapore) for 30 min at room temperature. Cells were seeded for experiments at a passage number of four. After pre-loading each culture chamber of the microdevices with 80 µl of medium, 20 µl of cell suspension (106 cells/ml) were manually injected in the wells of the culture chambers. During experiments, microdevices were kept in humidified chambers to avoid culture medium evaporation.

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Manufacturer protocol

Download the product protocol from Lonza for FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit below.

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