Advanced DMEM

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	Advanced DMEM supplemented with 100 IU/ml Penicillin, 1 mg/ml Streptomycin, 2mM L-glutamine and 5 wt.% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA)

Protocol tips
Advanced DMEM supplemented with 100 IU/ml Penicillin, 1 mg/ml Streptomycin, 2mM L-glutamine and 5 wt.% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA)

Publication protocol

The cartilage tissue was surgically removed under sterile conditions during TKA procedure. Distal and/or posterior femoral condyles were used for chondrocyte isolation depending on the macroscopic condition of the cartilage tissue (due to e.g., osteoarthritis). Immediately after the removal, the bone cuts were transferred into a previously sterilized 250 ml glass bottle filled with phosphate buffered saline (PBS; Sigma-Aldrich, Munich, Germany) and immediately brought to the cell isolation laboratory, and processed into a cell pellet.

The cell pellet was re-suspended in 20 mL of Advanced DMEM and centrifuged at 200× g for 5 min (Centrifuge 5804 R; Eppendorf, Hamburg, Germany). The supernatant was again carefully discarded and the cell pellet re-suspended in 10 mL Advanced DMEM supplemented with 100 IU/ml Penicillin, 1 mg/ml Streptomycin, 2mM L-glutamine and 5 wt.% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and plated on 25 cm2 flasks (in triplicates).

The culturing medium was changed every three days.

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