Smooth Muscle Cell Growth Medium 2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Protocol tips

Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Publication protocol

Human coronary artery smooth muscle cells were purchased from Promocell (Heidelberg, Germany) and cultured in Smooth Muscle Growth Medium Cell-2 containing 10% supplement-mix (PromoCell, Heidelberg, Germany). Cells were incubated at 37°C in a humidified atmosphere of 5% CO2. At the end of this period, the medium was removed, and cells were subjected to growth arrest in medium containing 0.1% supplement-mix before each experiment. HCASMCs between passages 3-5 were used for experiments and all explants stained positive for smooth muscle cell α-actin (Sigma, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Mammalian cell culture media HCASMC using Smooth Muscle Cell Growth Medium 2 from PromoCell.

Manufacturer protocol

Download the product protocol from PromoCell for Smooth Muscle Cell Growth Medium 2 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Mammalian cell culture media HCASMC using Smooth Muscle Cell Growth Medium 2 from PromoCell. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.