Endothelial Cell Medium

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	The cells were cultured and maintained in endothelial cell medium (ECM; cat. no. 1001; ScienCell Research Laboratories, Inc., Carlsbad, CA, USA) containing 5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin/streptomycin at 37°C in a humidified incubator (5% CO2). The experimental cells were treated with BTXA (0.1, 0.2, 0.4, 0.8, 1.6 or 3.2 U/ml) for 12 h before induction of hypoxia. Control cells were treated with PBS for the same period of time.

Protocol tips

The cells were cultured and maintained in endothelial cell medium (ECM; cat. no. 1001; ScienCell Research Laboratories, Inc., Carlsbad, CA, USA) containing 5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin/streptomycin at 37°C in a humidified incubator (5% CO2). The experimental cells were treated with BTXA (0.1, 0.2, 0.4, 0.8, 1.6 or 3.2 U/ml) for 12 h before induction of hypoxia. Control cells were treated with PBS for the same period of time.

Publication protocol

HDMECs were harvested from the upper eyelid tissues of 23 female patients with blepharoplasty (age range, 18–25 years) from October 2016 to November 2017. The cells were cultured and maintained in endothelial cell medium (ECM; cat. no. 1001; ScienCell Research Laboratories, Inc., Carlsbad, CA, USA) containing 5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin/streptomycin at 37°C in a humidified incubator (5% CO2). The experimental cells were treated with BTXA (0.1, 0.2, 0.4, 0.8, 1.6 or 3.2 U/ml) for 12 h before induction of hypoxia. Control cells were treated with PBS for the same period of time.

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