EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit

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	Human umbilical vein endothelial cells (HUVECs, Lonza, CC-2517A) were suspended in the recommended cell culture medium (basal media with growth factors, Lonza, CC-3162).

Protocol tips
Human umbilical vein endothelial cells (HUVECs, Lonza, CC-2517A) were suspended in the recommended cell culture medium (basal media with growth factors, Lonza, CC-3162).

Publication protocol

Human umbilical vein endothelial cells (HUVECs, Lonza, CC-2517A) were suspended in the recommended cell culture medium (basal media with growth factors, Lonza, CC-3162).

Approximately 106 cells in 2 ml of medium were introduced into each dish containing a set of collagen-coated rods. The cell culture medium was changed every day by adding 2–3 ml of fresh medium into the petri dish, mixing it well, removing the same amount, and repeating at least 3 times. The cells generally began to spread on the rods after one day and if the coverage was about 60%, and reached confluence after 3–4 days. For control experiments in 2D, glass bottom petri dishes (BD, FD35PDL-100) were plasma treated for 30 seconds, coated with 150 μg ml−1 type I collagen (BD, 354236) solution in 0.02 M acetic acid for 1–2 hours, and washed 3 times with PBS and once in cell culture medium prior to seeding with cells.

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Manufacturer protocol

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