Minimum Essential Medium Eagle

Mammalian cell culture media RAOSMC

Experiment
Mammalian cell culture media RAOSMC
Product
Minimum Essential Medium Eagle from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma).

Publication protocol

A7r5 rat smooth muscle cells were obtained from the ATCC (Manassas, VA). Bovine aortic endothelial cells (BAOECs), bovine aortic smooth muscle cells (BAOSMCs), and rat aortic smooth muscle cells were obtained from Cell Applications, Inc. (San Diego, CA). Commercially available cells were grown as recommended by the supplier and exchanged into minimum Eagle's medium over time before experiments. Human brain microvascular endothelial cells were obtained from Cell Systems (Kirkland, WA) and cultured using Cell Systems complete medium before exchange into minimum Eagle's medium supplemented identically as the BAOEC culture. All vascular cells were cultured on plates or coverslips coated with 0.2% porcine gelatin (Sigma).

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Manufacturer protocol

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