Mannitol Salt Agar

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	We obtained isolates from four sources: UTI [11], pregnant women's microbiota, minas cheese, and beach waters. We considered as suspect of S. saprophyticus the colonies from cultures of cheese and the microbiota samples that grew on Mannitol Salt Agar (BD, New Jersey, USA) with 100 µg/mL of novobiocin (Inlab, São Paulo, Brazil) and the colonies from beach water samples that grew on the same selective medium with addition of 0.005% sodium azide.

Protocol tips

We obtained isolates from four sources: UTI [11], pregnant women's microbiota, minas cheese, and beach waters. We considered as suspect of S. saprophyticus the colonies from cultures of cheese and the microbiota samples that grew on Mannitol Salt Agar (BD, New Jersey, USA) with 100 µg/mL of novobiocin (Inlab, São Paulo, Brazil) and the colonies from beach water samples that grew on the same selective medium with addition of 0.005% sodium azide.

Publication protocol

We obtained isolates from four sources: UTI [11], pregnant women's microbiota, minas cheese, and beach waters. We considered as suspect of S. saprophyticus the colonies from cultures of cheese and the microbiota samples that grew on Mannitol Salt Agar (BD, New Jersey, USA) with 100 µg/mL of novobiocin (Inlab, São Paulo, Brazil) and the colonies from beach water samples that grew on the same selective medium with addition of 0.005% sodium azide.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Bacterial cell culture media Staphylococcus saprophycitius using Mannitol Salt Agar from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for Mannitol Salt Agar below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Bacterial cell culture media Staphylococcus saprophycitius using Mannitol Salt Agar from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.