Cell Lysis Buffer (10X)

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	Proteins were extracted from cultured cells using lysis buffer (Cells Signaling Technology, Danvers, MA, United States).

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Proteins were extracted from cultured cells using lysis buffer (Cells Signaling Technology, Danvers, MA, United States).

Publication protocol

Proteins were extracted from cultured cells using lysis buffer (Cells Signaling Technology, Danvers, MA, United States). Equal amounts of proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Milipore, Billerica, MA, United States). The membranes were blocked with 5% nonfat milk diluted in Tris-buffer saline containing of Tween-20 (TBST) for 1h at room temperature before the addition of the appropriate primary antibody. The membranes were then washed with TBST and incubated with the appropriate HRP-conjugated secondary antibody (1:10000; Abcam, Cambridge, United Kingdom) for 1h at room temperature. Protein-antibody complexes were visualized using a chemiluminescence reagent (New England Nuclear, Boston, MA, United States).

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