RIPA Buffer

Protein isolation Mammalian cells - 3T3-L1

Experiment
Protein isolation Mammalian cells - 3T3-L1
Product
RIPA Buffer from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
After treatment with spilanthol, the cells were lysed in protein lysis buffer (Sigma, St. Louis, MO, USA). Protein samples (10–30 μg) were separated on 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF; Millipore, Billerica, MA, USA).

Publication protocol

After treatment with spilanthol, the cells were lysed in protein lysis buffer (Sigma, St. Louis, MO, USA). Protein samples (10–30 μg) were separated on 10% SDS polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF; Millipore, Billerica, MA, USA).

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Manufacturer protocol

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