CelLytic™ MT Cell Lysis Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cells or tissues were harvested in ProteoJET mammalian cell lysis reagent (Fermentas Life Sciences, Ontario, Canada) or CelLytec MT mammalian tissue lysis-extraction reagent (Sigma) with proteinase inhibitor cocktail (Roche), and protein concentrations were determined with a BCA kit (Pierce, Rockford, IL).

Protocol tips
Cells or tissues were harvested in ProteoJET mammalian cell lysis reagent (Fermentas Life Sciences, Ontario, Canada) or CelLytec MT mammalian tissue lysis-extraction reagent (Sigma) with proteinase inhibitor cocktail (Roche), and protein concentrations were determined with a BCA kit (Pierce, Rockford, IL).

Publication protocol

Cells or tissues were harvested in ProteoJET mammalian cell lysis reagent (Fermentas Life Sciences, Ontario, Canada) or CelLytec MT mammalian tissue lysis-extraction reagent (Sigma) with proteinase inhibitor cocktail (Roche), and protein concentrations were determined with a BCA kit (Pierce, Rockford, IL).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Mammalian cells - Rat_Mesenteric fat using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for CelLytic™ MT Cell Lysis Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Mammalian cells - Rat_Mesenteric fat using CelLytic™ MT Cell Lysis Reagent from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.