BMP-2 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use.	-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Publication protocol

In order to determine whether hBMP-2 was being expressed and to track the relative quantity of the protein over the designated culture period, an ELISA was performed. Briefly, by using a Quantikine ELISA Kit (R&D Systems), media were added into ELISA plates and hBMP-2 was then bound by pre-coated specific antibody. Afterwards an enzyme-linked monoclonal antibody specific for hBMP-2, followed by a substrate solution, were added to form an antibody-enzyme-substrate polymer. The color intensity of each well was measured using a Synergy HT microplate reader (BioTek). Media collected from the non-transduced adipose tissue discs cultured in normal medium and osteogenic medium served as controls. Nine samples per group were used for assay and measurements were performed in triplicate. The amount of BMP-2 produced by 4 adipose tissue specimen per well was presented.

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Manufacturer protocol

Download the product protocol from R&D Systems for BMP-2 Quantikine ELISA Kit below.

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