Human BMP-2 PicoKine™ ELISA Kit

ELISA Human - BMP-2

Experiment
ELISA Human - BMP-2
Product
Human BMP-2 PicoKine™ ELISA Kit from BosterBio
Manufacturer
BosterBio

Protocol tips

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The BMP-2 gene was delivered to hUCB-MSC. At 24 hours after transfection, the supernatant was collected for testing BMP-2 expression. Levels of BMP-2 production were evaluated by ELISA using corresponding anti-mouse antibodies and biotinylated secondary antibodies according to the manufacturer's instructions.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Human - BMP-2 using Human BMP-2 PicoKine™ ELISA Kit from BosterBio.

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Manufacturer protocol

Download the product protocol from BosterBio for Human BMP-2 PicoKine™ ELISA Kit below.

Download PDF Download manufacturer protocol

Videos

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