ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

ELISA Human - GDNF

Experiment
ELISA Human - GDNF
Product
ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Upstream tips
-Maintain the unopened kit at 2-8 C.
Protocol tips
-Vigorous washing and complete removal of all liquid by aspiration at the end of each washing step is very important to obtain low background values.
-Dilute the reagents immediately before use.
Downstream tips
-Do not store diluted solutions.
- Maintain the BDNF Standard, at 2-8 C for up to 30 days after reconstitution.

Publication protocol

Serum levels of total BDNF were measured by using six different ELISA kits, as listed in Table 2: human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF RapidTM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKineTM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex®/xMAP® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA). All samples were assayed in duplicate on each plate, in order to test also the intra-assay variation. A number of two plates per kit, of the same lot, were used over two different days, within the same week, in order to assess the inter-assay variation. The third replica for Aviscera-Bioscience, Biosensis and Millipore-ChemKineTM kits was carried out at 1 year distance from the second and therefore, was performed using a different lot. Protocols were performed according to the manufacturer’s instructions. The optical density of each well was measured using an automated microplate reader (GloMax®-Multi Microplate Reader, Promega) or the Bio-Plex®-200 instrument (Bio-Rad) for the multiplexing assay measurements.

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Manufacturer protocol

Download the product protocol from Merck Millipore for ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA below.

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