Human Total HO-1/HMOX1 DuoSet IC ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
--DO NOT FREEZE Streptavidin-HRP. -Bring all reagents to room temperature before use.	- Wash Buffer should be dispensed forcefully and removed completely from the wells by aspiration or decanting. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels. -Avoid microbial contamination of reagents and buffers. This may interfere with the sensitivity of the assay. - Determine the optical density of each well immediately after adding STOP solution.

Upstream tips
--DO NOT FREEZE Streptavidin-HRP. -Bring all reagents to room temperature before use.
Protocol tips
- Wash Buffer should be dispensed forcefully and removed completely from the wells by aspiration or decanting. Remove any remaining Wash Buffer by inverting the plate and blotting it against clean paper towels. -Avoid microbial contamination of reagents and buffers. This may interfere with the sensitivity of the assay. - Determine the optical density of each well immediately after adding STOP solution.

Publication protocol

Total HO-1 human ELISA DuoSet (R&D Systems DYC3776) was used to assay PTEC HO-1 protein expression. HO-1 protein measurements were adjusted for total protein concentration. To minimize inter-plate variation, HO-1 protein measurements were expressed as ratio of AngII-to-control for each plate. Mouse HO-1 ELISA kit (USCN Life Science Inc. E90584Mu) was used to measure urine HO-1 protein levels. Urine samples were prepared and assayed as per manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Total HO-1/HMOX1 DuoSet IC ELISA below.

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