Human Myeloperoxidase ELISA Kit (MPO) (ab119605)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

50 μL of supernatant collected following cell mixing treatment was assayed for GM-CSF (Ebioscience, cat# 88-8837 or Abcam, cat# ab174448). 1 μL of supernatant collected after cell mixing treatment was assayed for C3 (Abcam, cat# 108823); human plasma diluted 800× was assayed for C3 (Abcam, cat# 108822); murine plasma was diluted 50,000× (Abcam, cat# 157711); human plasma diluted 100× was assayed for Neutrophil Elastase (Abcam, cat# ab119553), MPO (Abcam, cat# ab119605), and histone nucleosome core (NovusBio cat# KA1091). 10 μL of plasma isolated after ex vivo treatment and 5 μL (20 μL for platelets only) of supernatant collected after cell mixing treatment were assayed for MPO (Abcam, cat# ab119605); 50 μL of plasma after ex vivo treatment was assayed for IFN-alpha (Abcam, cat# ab213479); 40 μL of plasma after ex vivo treatment was assayed for IL-8 (Ebioscience cat# 88-8086-22); and 40 μL of plasma after ex vivo treatment was assayed for citrullinated H3 (Cayman Chemical, MI, USA, cat# 501440); human plasma of influenza-infected patients and platelets supernatants were diluted 25×, and assayed for CCL5 (Abcam, cat# 174446). All procedures were performed according to the manufacturers’ instructions.

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Manufacturer protocol

Download the product protocol from Abcam for Human Myeloperoxidase ELISA Kit (MPO) (ab119605) below.

Download manufacturer protocol

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