Human NRG1 beta 1 ELISA Kit (ab100614)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

NRG1 beta 1 Human ELISA Kit (#ab100614) was purchased from Abcam (Cambridge, UK). Cell culture supernatant was collected from 6 wells 24 hours after seeding. ELISA was performed according to the manufacturer’s instruction. In brief, coated ELISA plates were incubated with 100μl either un-diluted cell culture supernatants or un-diluted sera from patients and NRG1 beta 1 standard diluted according to the manufacturer’s instructions for 2.5 hours. Solution was discarted and wells were washed for 5 times with 200μl 1x Wash Solution. Afterwards, 100μl 1x Biotinylated NRG1 beta Detection Antibody was added and incubated for 1 hour. After an additional wash step, which was performed as outlined above, 100μl 1x HRP-Streptavidin solution was added and kept at room temperature for 45 minutes with gentle shaking. After one further wash step (see above) the wells were incubated with 100μl TMB One-Step Substrate Reagent for 30 minutes in the dark with gentle shaking. Reaction was stopped by addition of 50μl Stop Solution. Measurement followed immediately at 450nm.

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Manufacturer protocol

Download the product protocol from Abcam for Human NRG1 beta 1 ELISA Kit (ab100614) below.

Download manufacturer protocol

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