Human Prolactin PicoKine™ ELISA Kit

ELISA Human - PRL

Experiment
ELISA Human - PRL
Product
Human Prolactin PicoKine™ ELISA Kit from BosterBio
Manufacturer
BosterBio

Protocol tips

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

CM from cultured hESCs was collected at day 5 of decidualization. PRL (Boster Immunoleader) and IGFBP1 (Raybiotech) concentrations were assayed by using commercial ELISA kits according to the manufacturers’ instructions.

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Papers

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Manufacturer protocol

Download the product protocol from BosterBio for Human Prolactin PicoKine™ ELISA Kit below.

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Videos

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