Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit

ELISA Rat - Serpin E1/PAI-1

Experiment
ELISA Rat - Serpin E1/PAI-1
Product
Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit from Cusabio
Manufacturer
Cusabio

Protocol tips

Upstream tips
Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay.
Protocol tips
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate.

Publication protocol

Culture medium samples were collected and stored at -80°C. PAI-1 levels in the medium were determined using the rat PAI-1 ELISA (Cusabio Biotech, China).

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Papers

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Manufacturer protocol

Download the product protocol from Cusabio for Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit below.

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