Active rat PAI-1 functional assay ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Do not freeze-thaw the standard and primary antibody more than once.	-Remove excess wash by gently tapping plate on paper towel or by kimwipe. -DILUTIONS FOR THE STANDARD CURVE AND ZERO STANDARD MUST BE MADE AND APPLIED TO THE PLATE IMMEDIATELY.

Upstream tips
-Do not freeze-thaw the standard and primary antibody more than once.
Protocol tips
-Remove excess wash by gently tapping plate on paper towel or by kimwipe. -DILUTIONS FOR THE STANDARD CURVE AND ZERO STANDARD MUST BE MADE AND APPLIED TO THE PLATE IMMEDIATELY.

Publication protocol

Plasma levels of rat active tPA and active PAI-1 were determined by ELISA (Molecular Innovations, Novi, MI). Plasma levels of rat ATIII, α2-MG, and soluble TM were measured by ELISA (Kamiya Biomedical, Tukwila, WA). Plasma levels of rat TFPI and TAFI were measured by ELISA (CUSABIO, Wuhan, PR China). Plasma levels of rat D-dimer was measured by ELISA (USCN Life Science, Wuhan, PR China). All ELISAs were run according to the manufacturer's procedures.

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Manufacturer protocol

Download the product protocol from Molecular Innovations for Active rat PAI-1 functional assay ELISA kit below.

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