Mouse Decorin ELISA Kit (DCN) (ab155454)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Discard the working standard dilutions after use – they do not store well.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Discard the working standard dilutions after use – they do not store well.

Publication protocol

For ELISA, blood was collected from fed and fasted mice and placed in an Eppendorf tube coated with EDTA to prevent coagulation. The whole blood was centrifuged for 5 min at 10,000 rpm and the plasma fraction was collected for analysis. Plasma decorin levels were determined using a standard mouse decorin ELISA kit following the manufacturer’s protocol (Abcam, ab155454).

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Manufacturer protocol

Download the product protocol from Abcam for Mouse Decorin ELISA Kit (DCN) (ab155454) below.

Download manufacturer protocol

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