SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Once in solution, store 1M DTT at -20°C.	-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed. -For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Upstream tips
-Once in solution, store 1M DTT at -20°C.
Protocol tips
-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed. -For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration. -For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Publication protocol

ChIP was performed using SimpleChIP Plus Enzymatic chromatin IP Kit (Agarose Beads) (Cell Signaling, CAT9004) according to the manufacturer's recommendations. Cells were fixed with formaldehyde and lysed, and chromatin was fragmented by partial digestion with Micrococcal Nuclease tomatic fragmentation to obtain chromatin fragments of 1 to 5 nucleosomes. Chromatin immunoprecipitations were performed using the MBDs, 10 μl of MeCP2, MBD3 antibodies and ChIP-Grade Protein G Agarose Beads. After reversal of protein-DNA cross-links, the DNA was purified using DNA purification spin columns.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 below.

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