SimpleChIP® Plus Sonication Chromatin IP Kit #56383

ChIP Human - AGS

Experiment
ChIP Human - AGS
Product
SimpleChIP® Plus Sonication Chromatin IP Kit #56383 from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Upstream tips
-Once in solution, store 1M DTT at -20°C.
Protocol tips
-For optimal ChIP results, use approximately 4 X 106 cells for each immunoprecipitation to be performed.
-For optimal ChIP results, it is highly critical that the chromatin is of appropriate size and concentration.
-For optimal ChIP results, use approximately 5 to 10 µg of digested, cross-linked chromatin per immunoprecipitation.

Publication protocol

SimpleChIP® Plus Sonication Chromatin IP Kit (#56383, CST) was used to detect the binding of the corresponding proteins to DNA. All experimental procedures were performed according to the instructions. Antibodies used were: LIN28A (Abcam), BMAL1 (CST).

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for SimpleChIP® Plus Sonication Chromatin IP Kit #56383 below.

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