ChIP Kit Magnetic - One Step (ab156907)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Keep away from light.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Upstream tips
-Keep away from light.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -An optimal chromatin amount is 5-10 µg per reaction. -Freshly prepared chromatin can be used directly for the reaction. Frozen chromatin samples should be thawed quickly at RT and then placed on ice before use.

Publication protocol

ChIP assays were conducted in SMMC-7721 and HepG2 cells following the manufacturer’s protocols (Abcam). Cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Following lysis and sonication, the supernatant was obtained by centrifugation. Samples were incubated overnight with anti-HOXA10 (Abcam) or control IgG (Abcam) in the presence of protein A/G beads. After washing, the enrichment of specific DNA sequences was measured by qRT-PCR.

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Manufacturer protocol

Download the product protocol from Abcam for ChIP Kit Magnetic - One Step (ab156907) below.

Download manufacturer protocol

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