BD Tryptic Soy Agar

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	Staphylococcus aureus USA300 LAC strain, a clinical isolate [14, 15], and Enterococcus faecalis OG1RF, a human isolate [16], were adopted as recipients for plasmid transfer experiments. All strains except E. faecalis OG1RF were primarily cultured in tryptic soy agar (TSA; BD Diagnostic Systems, Sparks, MD, USA) and tryptic soy broth (TSB; BD Diagnostic Systems) at 30 °C for 24 h to maintain their phenotypic traits. E. faecalis OG1RF traits were maintained in De Man-Rogosa-Sharpe (MRS; BD Diagnostic Systems) media at 30 °C for 24 h.

Protocol tips

Staphylococcus aureus USA300 LAC strain, a clinical isolate [14, 15], and Enterococcus faecalis OG1RF, a human isolate [16], were adopted as recipients for plasmid transfer experiments. All strains except E. faecalis OG1RF were primarily cultured in tryptic soy agar (TSA; BD Diagnostic Systems, Sparks, MD, USA) and tryptic soy broth (TSB; BD Diagnostic Systems) at 30 °C for 24 h to maintain their phenotypic traits. E. faecalis OG1RF traits were maintained in De Man-Rogosa-Sharpe (MRS; BD Diagnostic Systems) media at 30 °C for 24 h.

Publication protocol

Staphylococcus aureus USA300 LAC strain, a clinical isolate [14, 15], and Enterococcus faecalis OG1RF, a human isolate [16], were adopted as recipients for plasmid transfer experiments. All strains except E. faecalis OG1RF were primarily cultured in tryptic soy agar (TSA; BD Diagnostic Systems, Sparks, MD, USA) and tryptic soy broth (TSB; BD Diagnostic Systems) at 30 °C for 24 h to maintain their phenotypic traits. E. faecalis OG1RF traits were maintained in De Man-Rogosa-Sharpe (MRS; BD Diagnostic Systems) media at 30 °C for 24 h.

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