Nutrient agar

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25°C.	-Final pH 6.8 +/- 0.2 at 25°C. - If desired, the medium can be enriched with 5-10% v/v sterile defibrinated blood.

Upstream tips
-Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25°C.
Protocol tips
-Final pH 6.8 +/- 0.2 at 25°C. - If desired, the medium can be enriched with 5-10% v/v sterile defibrinated blood.

Publication protocol

P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) type strains obtained from the American Type Culture Collection were used throughout. Stock cultures were maintained on Nutrient Agar (NA; Sigma-Aldrich, USA) slants, and sub-cultured onto freshly prepared NA plates and incubated at 35°C for 24 h. For all planktonic, adhesion, and biofilm assays, NB (Sigma-Aldrich), BHI (HiMedia, India) broth, LB broth, and RPMI 1640 (Gibco, USA) medium were used as culture media.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Nutrient agar below.

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