CHARCOAL AGAR

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-pH 7.4 ± 0.2 @ 25°C. -Suspend 51g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, add 10% of defibrinated blood and mix gently. The medium is made selective for the isolation of Bordetella pertussis and Bordetella parapertussis by the addition of Bordetella Selective Supplement SR0082.

Protocol tips
-pH 7.4 ± 0.2 @ 25°C. -Suspend 51g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C, add 10% of defibrinated blood and mix gently. The medium is made selective for the isolation of Bordetella pertussis and Bordetella parapertussis by the addition of Bordetella Selective Supplement SR0082.

Publication protocol

B. pertussis isolates were cultured on charcoal agar plates (Oxoid England) containing defibrinated sheep blood at 10% and incubated at 35°C up to 7 days, and inspected daily. The isolates were stored at -80°C in medium containing brain heart infusion (BHI, Oxoid England) supplemented with 15% glycerol.

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Manufacturer protocol

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