Neogen-Brain-Heart Infusion Broth

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store dehydrated culture media at 2 – 30°C away from direct sunlight. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.	-The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms.

Upstream tips
-Store dehydrated culture media at 2 – 30°C away from direct sunlight. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect from moisture and light by keeping container tightly closed.
Protocol tips
-The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms.

Publication protocol

The MIC of streptomycin for S. enterica serovars was determined using micro-titer plate dilution method after taking spectrophotometric absorbance measurements at 595 nm [37] with micro-titer plate reader (Benchmark, BioRad). The MIC of streptomycin was tested with 0–2000 μg/mL concentration of antibiotic. An inoculum of around 106 cells of each strain resuspended in LB broth was added in 200 μL reaction volume and the plates were incubated at 37°C and 70 rpm for 24 h. To find the streptomycin-sensitive strains from the top 20 human-origin Salmonella serovars, the assay was performed in BHI broth supplemented with 100 μg/mL streptomycin (Strep100).

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Manufacturer protocol

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