Quick Amp Labeling Kit-one color

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Store entire kit at room temperature. After breaking foil on microarray pouch, store microarray slides at room temperature (in the dark) under a vacuum dessicator or nitrogen purge box. Do not store microarray slides in open air after breaking foil.

Upstream tips
Store entire kit at room temperature. After breaking foil on microarray pouch, store microarray slides at room temperature (in the dark) under a vacuum dessicator or nitrogen purge box. Do not store microarray slides in open air after breaking foil.

Publication protocol

RNAs obtained from cells were amplified and labeled starting from 150 ng and using the Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies) as described by the proprietary protocol. Labeled cRNA was purified with miRNeasy Mini Kit (QIAGEN) and quantified using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). On average, cRNA yield was about 10 μg and the specific activity was of 35 pmol Cy3 per μg cRNA.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Quick Amp Labeling Kit-one color below.

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