Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-It is highly critical that the chromatin is of appropriate size and concentration.	-It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date.	-Once in solution, store 1M DTT at -20°C.

Upstream tips
-It is highly critical that the chromatin is of appropriate size and concentration.
Protocol tips
-It is important to keep the tissue cold to avoid protein degradation. -Use fresh formaldehyde that is not past the manufacturer's expiration date.
Downstream tips
-Once in solution, store 1M DTT at -20°C.

Publication protocol

Chromatin-IP was performed using the ChIP Assay Kit (Millipore) according to the manufacturer’s protocol by using anti-H3 (cat. #14269), anti-CENP-A (cat. #2186), anti-H3K4me1 (cat. #5326), anti-H3K4me2 (cat. #9725), and anti-H3K4me3 (cat. #8580). All antibodies are from Cell Signaling. Real-time PCR was performed to detect relative occupancy with the primers for chromosome 1 a-satellite DNA purchased from Cell Signaling.

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 below.

Download manufacturer protocol

Videos

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