QIAGEN Large-Construct Kit

Plasmid Isolation DH10Bac (Bacmid)

Experiment
Plasmid Isolation DH10Bac (Bacmid)
Product
QIAGEN Large-Construct Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Starting material <500 ml culture volume

Publication protocol

The construct was transformed into DH10Bac E. coli (Invitrogen) where the Igκ-T2 insert was spontaneously transposed into bacmid. The resultant recombinant bacmid designated BacIgκ-T2 was purified by Large-Construct Kit (Qiagen, Hilden, Germany) and transfected into Sf9 cells using Cellfectin reagents (Invitrogen).

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for QIAGEN Large-Construct Kit below.

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