Plasmid Mini

Plasmid Isolation Corynebacterium diphtheriae

Experiment
Plasmid Isolation Corynebacterium diphtheriae
Product
Plasmid Mini from A&A Biotechnology
Manufacturer
A&A Biotechnology

Protocol tips

Protocol tips
Starting material from 1.5–3 mL cultures

Publication protocol

Two methods were used for plasmid isolation: alkaline lysis according to Sambrook and Russel14 and a commercial kit – Plazmid Mini (A&A Biotechnology, Poland). As a control we used Escherichia coli V517 strain, which possesses eight cryptic plasmids
of different sizes.

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Papers

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Manufacturer protocol

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