HiSpeed Plasmid Kits

Plasmid Isolation Enterobacteriaceae-E. coli transconjugate

Experiment
Plasmid Isolation Enterobacteriaceae-E. coli transconjugate
Product
HiSpeed Plasmid Kits from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Starting material 50 ml – 150 ml culture volume

Publication protocol

Then plasmid DNA was extracted from the transconjugant colonies using a HiSpeed plasmid maxikit (Qiagen). E. coli DH5α cells were transformed with the extracted plasmid. The Etest dry-strip technique was used to determine the hydrolytic activities of the J53 transconjugants and DH5α transformants.

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Manufacturer protocol

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