p-c-Jun Antibody (KM-1): sc-822

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Protocol tips
-Mouse (1:1000) -Use clean forceps to gently handle the blot from the corner without creasing the membrane. Do not write on the blot with pen or marker, as the ink can fluoresce and cause background.

Publication protocol

For western blot analysis, equal amounts of proteins were fractionated on appropriate percentage of SDS–polyacrylamide gel and transferred onto polyvinylidene difluoride membranes. After the transfer, the membranes were blocked with 5% nonfat dry milk in PBS containing 0.05% Tween 20 and probed with various primary antibodies. After incubation with secondary antibodies, immunoblots were visualized with the enhanced chemiluminescence detection kit (GE Healthcare). Reproducibility was confirmed in three separate experiments and representative data were shown.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for p-c-Jun Antibody (KM-1): sc-822 below.

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