MMP9 Monoclonal Antibody (IA5)

Western blotting MMP-9

Experiment
Western blotting MMP-9
Product
MMP9 Monoclonal Antibody (IA5) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
-Mouse (1:500)

Publication protocol

Total membranes and nuclear extracts were prepared as described previously, and protein concentrations were determined using the BCA assay (Pierce, USA). The dilution of primary antibodies was as follows: MMP2 (MA1-772, 1:500); MMP9 (MA5-14220, 1:500); MMP13 (MA5-14247, 1:350); FSTL1 (PA5-31113, 1:1500); Rac1 (PA1-091, 1:1000); Cdc42 (PA1-092X, 1:1000); RhoA (MA1-011, 1:1000); TLR4 (MA5-16216, 1:1000); and NF-κB (PA1-186, 1:1000). All primary antibodies were detected with rabbit polyclonal antibodies (Invitrogen). Each membrane was rinsed three times for 15 min and incubated with the secondary horseradish peroxidase-linked antibodies. β-actin was used as the loading reference for data analysis.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MMP9 Monoclonal Antibody (IA5) below.

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