α-Tubulin Antibody #2144

Western blotting Tubulin alpha

Experiment
Western blotting Tubulin alpha
Product
α-Tubulin Antibody #2144 from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
-Rabbit (1:3000)

Publication protocol

Standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and an enhanced chemiluminescent (ECL) substrate (ThermoFisher, Cat# 32106) was applied except for STING dimerization assay where no reducing agent was applied to the samples. In some cases, such as K63-Ub, an ultra-sensitive ECL substrate for low-femtogram-level detection (ThermoFisher, Cat# 34095) was used.

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for α-Tubulin Antibody #2144 below.

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